As the previous poster said, no 1-bedroom villas on property have 2 bathrooms.
Yes there is a door seperating the main bedroom from the living area. There is no door seperating the tub/sink area of the main bedroom though, simply a wall with a "window-like" cut out. A bathroom with a toilet, additional sink, and shower is seperated by a door from the sink/tub area of the room.
This is slightly different compared to other 1-bedroom villas on property which have an additional door in the toilet/sink/shower bathroom that opens directly to the main living area. This came in handy when using the sleeper sofa so that guests didn't have to go through the main bedroom to use the toilet/sink/shower.
If two bathrooms are important to you, I suggest looking into the Family Suites at Disney's All Star Music. These have a similar set up to the 1-bedroom villas (seperate living area, seperate bedroom with Queen bed), but come with two full bathrooms. Since these were converted from standard guest rooms, the suites do not have full kitchens, but rahter a small kitchenette area like you'd find in the studio villas with slightly more counter and shelf space.