i have been to por three times now and love it!! it is my favorite!! i know building 14 in allegator bayow does accessible rooms. i had to call disney directly because i have a relative that is in need of a roll in shower. we got one accessible room for my relatives and a contecting room for my family of four in building 14. all of allegator bayow has trundle beds. i don't think the accessible rooms have trundle beds but the other rooms in that building should. to double check on this you could visit the portorleans unofficial website ( i think at portorleans.org), or call the resort directly.
hope this helps,
lisa