I have stayed at both the Amerisuites and the Holiday Inn at Universal- no comparison-
Holiday Inn wins hands down!!!
My suite there was very nice- a TRUE suite with 2 large rooms both with TV's, and a full sized kitchenette, as someone else described earlier- I was comfy there with my 2 kids, and the last night even had a friend and her hubby sleep on the queen sized convertible couch.
The amerisuites, on the other hand was a complete disaster start to finish- The "suite" is actually a standard sized hotel room with the 2 double (not queen) beds pushed to within a foot of each other and one bed right up against the window a/c unit. This allows for them to put up a half wall about 10 feet into the room into which the stuff a small chair, a loveseat, a desk and a counter with a mini-fridge (not even large enough to stand a 2 liter coke bottle in), a microwave, and a sink too small to wash a dinner plate. There is only one TV on a swivel base so you can aim it either the sitting area or the beds.
Add to this a fire alarm going off at 4am (thank God it was a false alarm- we got quite a jammie fashion show in the lobby!), no shower curtain, returning from the park at 11pm to find our door had been left propped open all day by housekeeping, finding an empty vodka bottle behind the loveseat, and a totally indifferent staff... well you get the idea- avoid amerisuites at all costs!
Hope this helps!