Whether the single line is open or not usually depends on the stand by wait time. If it's not under 60 minutes, you probably won't see the single line open.
We got to ride it 3 times on March 9th. We had Express passes with our on-site hotel key card. I lovethe rides at IoA and Universal!
DD (age 9) is not even a Spiderman fan (she's a girly girl). She went nuts over that ride. And she is afraid of roller coasters but likes the virtual thrill rides. Spiderman is just perfect. Those of you about to go to IoA are going to have so much fun. I wish we could go again!