Test track has one and I think MS still has one. And as Hope73 stated above EE has single rider. I think thats all, I just read somewhere that its only offered at those three places.
I know that a lot of rides don't have single rider lines, but I am famous for thinking that I'll ride something and then run at the last minute. This happens for the Tower of Terror. I rode it once and never again...I know that for this ride when I walk through with DH and take the 'sissy elevator' down they always ask once you get closer if there are any single riders. I've seen this done on a few other rides too. You'll be fine...or maybe you'll meet a riding buddy in line.
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