If you are using the Gif file I did for you, there is no background on the image, but I am not to fimiliar with dark transers as to how they print gifs. Matter of fact I have never used dark transfers before, so I am not sure if the dark transfers will put a white background or not, but I do know that the original image has no background so with the white transfers it would be clear. My best advise to you is to post a request on the main board regarding dark transers and GIF files if they print with white background. I don't want you to risk it because I am not positive. I received a PM from M_I_C_K_E_Y and she is the expert on transfers of all kinds and she said I got mixed up with my advise about dark transfers so maybe consult with her first.
"If you use a light transfer, the background and any white in the design is clear (the shirt color) and if you use a dark transfer the background and all white in the design are white." is what she sent me.But I don't know how this relates to GIF files so maybe we should get clairification first.
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If you use dark transfer paper the background will be white. If you use light transfer paper you will need to flip the design when you print and the background will be clear (the shirt color) and any white in the design will also be the shirt color.
I have done GIF file on dark transfer paper and since the paper is white the background is white.
Hope that helps.
Tracie