Short Answer - Yes, I've always worn my wedding rings on vacation.
Long Answer -
My first set, I wore all the time until I got pregnant w/ our first DD. My fingers got bigger, & my wedding band would no longer fit. My grandfather gave me my grandmother's band to wear to w/ my engagement ring.
After my DD was born, I continued to wear just my grandmother's band because I didn't want the diamond from my engagement ring to scratch my baby. (It was a pear-shaped solitaire diamond that stuck up from the band.)
18 months after she was born, her little brother was born... so I just continued wearing my grandmother's band.
But I wore the band all the time, &, after the kids wore older, I started wearing my engagement ring again. My original wedding band fit again, but, since I really liked wearing my grandmother's band, I wore all 3 (thin bands).
And, I wore them all the time.
For our 17th wedding anniversary, DH gave me a new set. According to the care instructions, you're not supposed to wear your rings when you sleep, take a shower, swim, clean, etc. So, for a while, I took my rings off for any of those kinds of activities - including sleeping.
I'm a beach girl too, so, whenever we'd go to the beach, I'd take my rings off before we went to the beach & leave them in our room - but I'd still have them w/ me on vacation.
But, eventually, I got tired of taking my rings off all the time for the various things, & I was afraid I was going to lose them.
So, now, I only take them off if I'm doing something really messy or have them off in order to clean/inspect the rings.
Other than that, my rings are always on - even on vacation.