No. All routes take you to either DC or Pennsylvania and then have you change trains heading south. The trip normally takes about 2 days each way, allowing for delays (there almost always are delays.)
To make this trip, you will have to change trains in either Washington or New York. Easiest way would probably be the Capitol Limited to D.C., then transfer to the Silver Meteor south to Orlando or Kissimmee (closest station to WDW).
Union Station in Washington is a fairly nice place to spend a few hours between trains, though, so the connection isn't that bad.