ugh ... answering this again .... lots of BAD stuff has been provided (but well intentioned)
from Ft L to PC just take 95, every mile north the traffic will be less intimidating. If you try the turnpike you WILL get confused fast.
The port is on SR
528 .... NOT 520 .... the main port hotels are on 528 (Radisson, Country Inn, Residence Inn, Homewood)
going from the port to Tampa I recommend you go 528 to 417 to I-4
on 528 you will encounter two tolls, both are $1 and you can pay cash. Be aware, toll plazas around here are set up for electron payment to zip right thru with no slowdown. To pay cash you take what looks like an exit to the right. Here you find attended and exact change lanes. If there is gonna be a backup, and there can be a big one, it will be at the attended lane. A roll of quarters can see you thru all the tolls you will encounter (one way)
IMMEDATELY after the second 528 toll you exit right and get on 417 towards WDW and Tampa. As I recall you encounter 2 tolls of $1.50 and same deal as 528 wrt the cash lanes looking like exits. (correction,there is a third toll of $1 ...)
At the end of 417 you are on I-4 and no more tolls unless you get very lost in Tampa where there IS a small "crosstown expressway" that is a toll and an ELECTRONIC ONLY one at that (dangerous in a rental if not informed)
rental companies DO offer the option to handle electronic tolls for you, charging a service fee. What this costs varies WIDELY. AND what the rental car companies charge as a 'processing fee' if you make a booboo and accidentally drive thru "electronically" also VARIES WIDELY with some charging $15 per toll. (this is not FL btw ... it is the rental company)
I suggest reading ALL of
https://www.sunpass.com/rentalcar
welcome to Florida ... the rules are different here