To take it back what was being discussed was women who overtook the men's bathroom of which there were only 2 bathrooms total (one men's one women's) and then proceeded to not allow men into the men's bathroom. I disagreed with physically blocking someone (
anyone) from using the bathroom in that scenario and it's also far beyond what the OP was discussing. As far as let comment that came from this
"women commandeered the men’s room and prevented any men from entering" and
"I actually appreciated that the women were preventing men from entering the men’s room while women were in there."
As far as your quip about me talking about open minds. When I said presumed gender it was in response to you describing blocking men from using the men's bathroom because a woman was in there. You presumed that all those in line in the women's bathroom were identifying as biologically women of which there are more perspectives than that including non-binary and so much more. For instance a person in the midst of transitioning may wish to use the women's bathroom as their preference and they may be in a dress or they may be in a suit whatever strikes their fancy but that alone does not determine their gender identity.
There aren't any different circumstances here, we're talking about blocking access to the only bathroom for men available for usage. It is unreasonable to not expect men to use the men's bathroom. I could understand giving a heads up to any one person who comes up to the men's bathroom and the reverse if this was the case but to physically block no. There's nothing inherently different about individuals using the bathroom to relieve themselves.
Coming at it from the angle of what some have spoken about just what do you think the men were going to do in the men's bathroom? Maybe use the bathroom? And if that makes you (general you) so uncomfortable don't use the men's bathroom. No nefarious intentions, no alterative motives, etc are aimed at women in the situation you described if men went to use the men's bathroom.
I'm guessing you felt a certain way about this situation because it occurred at a wedding you attended, you may have known at least some of these people personally but out and about in the public you may not see men as accommodating nor may you find all women banding together to do such action, as I said I wouldn't partake in such action and would call out such behavior.
I've also got to say it's not unheard of for some of us to describe some men in our lives who spend way more time in the bathroom than we do

(I know several including my husband on average) but now we're getting into bodily functions and while we can go there I'm not sure we want that. For that you can see this thread
https://www.disboards.com/threads/people-
-in-line-at-wdw.3933859/