You can buy a SunPass mini transponder to stick on your windshield at the first Turnpike Service Plaza after you turn onto the Turnpike from I75. (which I think was undergoing an entire refurb when I went by in January). Or you can buy it on-line and they will mail it to you:
https://www.sunpass.com/index (see the link that says "Click Here to Purchase" and choose the mini).
I keep a minimum amount of money on it and automatically adds when balance gets low. I go to that part of Florida maybe once-twice a year and it's nice to roll through the toll booths like a local. The Florida Turnpike from Ocala/Wildwood down to about West Palm Beach (which includes the Orlando area) are still using manned booths or the SunPass. Hwy 429 that tiggerdad mentions, like some other toll roads around Florida that aren't officially part of the Turnpike system, use the SunPass or cash as well. Below West Palm, somewhere around Lauderdale there are no more manned toll booths on the Turnpike and it's Sunpass only.
Thing about the SunPass is to list BOTH the tow vehicle VIN/plate as well as the trailer VIN/plate. Even though my trailer is small, I like not having to come to a complete stop at a manned booth and then accelerate back up to speed. So if you're going no further than Orlando and won't be coming often, just pay the cash tolls at the manned booths. Or get the Mini and roll on through.
Bama Ed