Yes, a wheelchair can be loaded at all stations except Main Street. For Toon Town and Tomorrowland stations, go up the exit and wait there for the train. At New Orleans Square, it is also at the exit (at the front of the train) that you will wait. Once a train arrives, the conductor will come over and ask if you can transfer or if you need to ride in the chair. We have a manual chair and can fold it, but my Mom can't make the step right now, so we need the wheelchair compartment spot. Beware that if the wheelchair spot is full or someone in front of you needs it you will need to wait for the next train and that spot may not be available on the next one either. The wheelchair itself is not tied down --- just the brakes locked. It does not seem to move at all though.