no, they won't.
normally, when you make your reservation, you pay a one night stay at that time.
when you check out, you pay the remainder of the cost of your stay.
in ky07's situation, i have no idea why he paid both nights in advance at check in. since he booked thru AAA, maybe it was a procedure to do it that way.
i don't use a third party when i book for the UO onsite hotels.
i have stayed 21 times at the onsite hotels and have always settled my bill at the time of check out.