I think it depends on where you're coming from.
If you're traveling down I-75 then you'd take exit 329 to Florida's Turnpike (aka Ronald Reagan Turnpike) toward Orlando. Then you'd likely continue to either exit 267a (Western Expressway/Route 429) or exit 259 to go onto I-4. I'm pretty sure that there are three lanes at the exit 259 toll plaza (the one on the left is attended and they'll make change for you, the middle lane is an exact change lane, and the right lane is the one that you will want to avoid - it's a Sun Pass only lane). I'm not familiar with the situation for exit 267a but I think that there's a toll plaza on the exit there, too. But 429 is also a toll road and I believe that the toll plaza is built as part of the regular roadway (if that's right then there's no veering off for the cash booths there). I think that that's the only toll booth until you get to the an exit (and I'm not sure that all of the exits have tolls).
Others on this post have talked about the pay lanes being separate from the Sun Pass straight through lanes on toll roads. I know that that's the case for SR 528 and SR 417 which are the east-west roads that are to the north and south, respectively, of the airport where most of us travel toward Disney from but if you're driving from an out-of-state location then these probably won't apply to you.
Dick Taylor