We do split stays a lot. Will you have a car?
What I like to do is pack everything we need for the night(s) consolidated into one or two bag/suitcases depending on how long the split stay is (there are usually four of us, so four suitcases generally). Makes it easier to bring in only what we need rather than digging around for what we need. If we have a car, the rest of the stuff just stays in the trunk until we move hotels. If we don't have a car, then we just set those suitcases off to the side for our second half of the stay.