Fun Spot is the first place that comes to mind. I was thinking they had two locations, but now I'm not so sure. I think there is a track at Old Town as well, but I don't know if it is any good.
Orlando Kart Center is a more serious Kart Track for Kart Owners. They also have rentals. They usually hold rental sessions for beginner/ novice drivers only on Sundays. It is located on Parcel Ln just west of the airport and south of the Beachline SR-528 http://orlandokartcenter.com/
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