Are you coming down I-95 from NC? If so, using 301 to cut across the state isn't a bad idea, but you'd want to take I-95 to I-295 north of Jacksonville (just past the airport), then onto I-10 west to Baldwin where you could get onto US-301 and take it all the way to Ocala to catch I-75 for the rest of the trip into Tampa. It cuts of some miles and avoids the heaviest traffic on I-4 around Orlando, but probably isn't necessarily a
faster trip
per se.
PM me if you have any questions.