if you have a standard room, it could be tight.
if you could push the 2 beds apart and put the blow up between the 2, it might work.
but, you wouldn't have much leeway for the one that had to get out of the bed at night for the bathroom, could step on one of the sleepers on the floor.
if you put the blow up in the entrance area outside of the bathroom, it's a possibility but tight fit for anyone getting up in the night.