If you mean at night when it's pitch dark outside, yes, we've done that (we've done sunset too). It's really awesome to see the place in the dark, it's an entirely different experience. I will say that from a "seeing animals" perspective, it wasn't as good simply because it was harder to spot them in the dark. There is some lighting, but it's still a bit of a challenge to see, especially if they're some distance away. I'd still recommend doing it, just so you can get a new perspective of the safari.
The sunset one is beautiful!