Yes, you can. When I was there this past Sept/Oct for the Food & Wine Festival I used a $500.00 Disney gift card that I had bought online from the Disney website. I used it at the F&W Festival booths, in the restaurants to pay for my tips, and once in the food court for a cookie, I didn't want to waste a snack credit on. I also used it in the gift shops. It was never turned down anywhere I tried to use it.