There was some discussion in another thread about Hwy 301/I-75/Florida Turnpike as an alternate route from Jacksonville to WDW:
http://www.disboards.com/showthread.php?t=1433835
I hate I-95/I-4, so 301/I75/Turnpike is now the route I take and I have been quite pleased with it, but as you will see in the above thread, another poster found it totally unacceptable. So, as always "your mileage may vary".
In general, I think if you don't hit rush hour in Jax or Orlando, and you don't hit any major accident or construction-induced traffic backups on I95 or I4, then the I95/I4 route has to be the quickest (and for most folks easiest) choice. However, since I always seemed to hit one of those backups or a rush hour, and since I'm the type that often prefers "back roads" to interstates, I'll be happily driving 301/I75/Turnpike again next month.